The list is made up of four goalkeepers, eleven defenders, ten midfielders and seven forwards.
Madrid, who are the defending champions, will kick off their Champions League campaign in two weeks against AS Roma at the Santiago Bernabeu.
The Los Blancos will also face CSKA Moscow and Viktoria Plzen in the Group Stage.
Below is Madrid’s Champions League squad:
GK: Keylor Navas, Thibaut Courtois, Kiko Casilla, Luca Zidane.
Defense: Dani Carvajal, Jesus Vallejo, Raphael Varane, Sergio Ramos, Nacho, Marcelo, Sergio Reguilon, Alvaro Odriozola, Adri, Javier Sanchez, Sergio Lopez.
Midfielders: Casemiro, Marcos Llorente, Toni Kroos, Luka Modric, Dani Ceballos, Isco Alarcon, Fede Valverde, Jaime Seoane, Franchu.
Forwards: Gareth Bale, Lucas Vazquez, Karim Benzema, Marco Asensio, Mariano Diaz, Vinicius Junior, Cristo Ramon Gonzalez Perez.